教育经历 / Education
1. 2004年9月 - 2009年12月 博士学位，神经科学，导师：何士刚研究员 中国科学院生物物理研究所，脑与认知科学国家重点实验室 2. 2006年12月 - 2008年1月 博士生交流项目 导师：Thomas Euler 教授，Winfried Denk 教授 马普医学研究所（德国）, 生物医学光学部 3. 2000年9月 - 2004年7月 学士学位，应用物理, 南开大学物理学院
1. Sep. 2004 - Dec. 2009 Ph.D. of Neuroscience Prof. Dr. Shigang He’s lab, State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China 2. Dec. 2006 - Jan. 2008 Doctoral training program Prof. Dr. Thomas Euler’s group, Department of Biomedical Optics (Prof. Dr. Winfried Denk in charge), Max Planck Institute for Medical Research, Heidelberg, Germany 3. Sep. 2000 - Jul.2004 B.S. of Applied Physics Institute of Physics, Nankai University, Tianjin, China. Thesis: “AFM image reconstruction by using the best-optimized tip geometry”
工作经历
1. 2021年3月-今，首都医科大学，北京脑重大疾病研究院，低氧适应医学研究平台，教授 2. 2012年10月 – 2021年2月，中国科学院生物物理研究所，助理研究员/副研究员 3. 2010年2月-2012年9月，中国科学院生物物理研究所，博士后
1. Mar. 2021 – Now Employer: Beijing Institute of Brain Disorders, Capital Medical University Position: Professor 2. Oct. 2012 – Feb. 2021 Employer: Institute of Biophysics, Chinese Academy of Sciences Position: Assistant / Associate investigator 3. Feb. 2010 - Sep. 2012 Employer: institute of biophysics, Chinese Academy of Sciences Position: Post Doc.
研究概述
课题组从细胞类别特异性的角度解析脑的功能构成，通过联合多种前沿技术手段解析细胞类型特异性环路在认知过程中的作用机制：利用转基因小鼠结合光遗传调控和病毒示踪，对不同细胞类别进行研究，阐明其功能及分子调控机制；使用基因表达标记结合patch-seq，将认知过程中的神经元功能与组学特征联系起来；依据分子表达特征，阐明特定认知过程相关神经网络中的细胞类别构成、环路连接样式、功能调控分子及神经编码机制，构建正常及疾病状态下的神经功能环路模型。课题组在分析单细胞功能及组学特征（Nature，2020；Protein Cell，2020）；荧光微镜解析动物行为（Science Bulletin，2020）；细胞形态重构及环路解析（PLOS Biology，2020；Crebral Cortex，2018）方面具有丰富的经验。
The research team analyzed the functional structure of the brain from the perspective of cell type specificity, and analyzed the mechanism of cell type specific circuits in the cognitive process by combining a variety of cutting-edge technologies: using transgenic mice combined with optogenetic regulation and virus tracing, to study different cell types, to clarify their functions and molecular mechanisms; to use specific molecular markers combined with patch-seq to link neuronal functions with omics characteristics; to clarify specific cognitive behavior based on molecular expression characteristics, the cell-type composition, circuits connection patterns, functional molecules and neural coding mechanisms in behavior-related neural networks. Our aim is to construct neural circuits model under normal and different disease states. The research group has extensive experience on analyzing single cell functions and omics characteristics (Nature, 2020; Protein Cell, 2020); visualization of behavior-related neuron activity in free-moving animal (Science Bulletin, 2020); reconstruction of cells morphology and their functional connections (PLOS Biology, 2020; Crebral Cortex, 2018) .
代表成果
一、发表论文 1. Zhong S#, Ding W#, Sun L#, Lu Y#, Dong H, Fan X, Liu Z, Chen R, Zhang S, Ma Q, Tang F, Wu Q*, Wang X*. 2020. Decoding the development of the human hippocampus. Nature 577:531-536. 2. Sun L#, Chen R#, Li L#, Yuan B#, Song K, Pan NC, Cheng T, Chang S, Lin K, He X, Wu Q, Xu F, Qiu Z*, Wang X*. 2020. Visualization and correction of social abnormalities-associated neural ensembles in adult MECP2 duplication mice. Science Bulletin. 65(14):1192-1202. 3. Shi Y#, Sun L#, Wang M#, Liu J#, Zhong S, Li R, Li P, Guo L, Fang A, Chen R, Ge W, Wu Q*, Wang X*. 2020. Vascularized human cortical organoids (vOrganoids) model cortical development in vivo. PLOS Biology 18(5):e3000705. 4. Liu J#, Wang M#, Sun L#, Pan NC#, Zhang C, Zhang J, Zuo Z, He S, Wu Q*, Wang X*. 2020. Integrative analysis of in vivo recording with single-cell RNA-seq data reveals molecular properties of light-sensitive neurons in mouse V1. Protein Cell. 11(6):417-432. 5. Fan X#, Fu Y#, Zhou X#, Sun L#, Yang M, Wang M, Chen R, Wu Q, Yong Y, Dong J, Wen L, Qiao J*, Wang X*, Tang F*. 2020. Single-cell transcriptome analysis reveals cell lineage specification in temporal-spatial patterns in human cortical development. Science Advances. 6(34):eaaz2978. 6. Sun L#,*, Chen R#, Bai Y, Li J, Wu Q*, Shen Q*, Wang X*. 2019. Morphological and Physiological Characteristics of Ebf2-EGFP-Expressing Cajal-Retzius Cells in Developing Mouse Neocortex. Cereb Cortex 29:3864-3878. 7. Li R#, Sun L#, Fang A#, Li P, Wu Q*, Wang X*. 2017. Recapitulating cortical development with organoid culture in vitro and modeling abnormal spindle-like (ASPM related primary) microcephaly disease. Protein Cell 8(11):823-833. 8. Yuan Q#, Deng KY#, Sun L#, Chi S#, Yang Z, Wang J, Xin HB*, Wang X*, Ji G*. 2016. Calstabin 2: An important regulator for learning and memory in mice. Sci Rep 6:21087. 9. Sun L#, Han X#, He S*. 2011. Direction-selective circuitry in rat retina develops independently of GABAergic, cholinergic and action potential activity. PLoS One 6:e19477.
二、专利 1. 王晓群; 吴倩; 李睿; 孙乐; 方艾; 一种类脑器官器的制备方法和应用, 2017-6-28, 中国, 201710512944.0. 2. 王晓群; 仇子龙; 吴倩; 孙乐; 一种改善自闭症社交障碍的方法, 2020-1-21, 中国, 202010068656.2.
